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GRAPHITE BASED BIENZYME SENSORS
Kulys, Juozas J. Bilitewski, Ursula Schmid, Rolf D.
Kulys, Juozas J.
Bilitewski, Ursula
Schmid, Rolf D.
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Issue Date
1992
Submitted date
2024-05-08
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Adobe PDF, 4.28 MB
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Abstract
Robust peroxidase/oxidase sensors based on both chemically modified and unmodified graphite electrodes were prepared. The sensorsacted atelectrode potentials of -0.2 V to 0.2 V vs Ag/AgCl electrode. The Sensors werespecific to glucose, alcohols, choline chloride, L- and D-amino acids and oxalic acid. The sensitivities of sensors ranged from 0.3 - 12 uA/mM and the determination ranges of metabolites were 0.01 - 20 mM. The concentration range of cholesterine determined using a hydrogen peroxide sensor and soluble cholesterol oxidase was 0.01 - 0.26 mM with a sensitivity of 0.25 mA/mM whendetermination was carried out using the in biocatalytic pre-concentration regime. The pH optima andthe longterm stabilities of the sensors depended largely on the properties of the oxidase immobilized
Citation
Biosensors : fundamentals, technologies and applications, 393 - 401
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
EISSN
ISBN
1560812206
3527284370
3527284370
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Attribution-NonCommercial-ShareAlike 4.0 International