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ISOLATION OF GLYCOPEPTIDES OF THE ENVELOPE GLYCOPROTEIN FROM A POLYTROPIC MURINE RETROVIRUS
Geyer, Hildegard Geyer, Rudolf
Geyer, Hildegard
Geyer, Rudolf
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Issue Date
1991
Submitted date
2024-02-20
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Abstract
A polytropic recombinant retrovirus containing the envelope gene of Friend mink cell Focus-inducing virus plus the remainder of the genome of an amphotropic murine leukemia virus was propagated on mink lung cells in the presence of F222 Hterenninaes Radiolabeled viral glycoprotein was isolated hy immunoaffinity chromatography and purified by preparative SDS polyacrylamide gel electrophoresis. Glycopeptides obtained after digestion with trypsin were fractionated by gel filtration and reversed-phase HPLC, and Oligosaccharides attached were characterized by their sensitivity towards endo-ß-N-acetylglucosaminidase H and glycopeptidase F and, in part, by chromatographic Procedures and methylation analysis. The results obtained Provide evidence for a specific glycosylation of the viral glycoprotein in mink lung cells with Tegard to the distribution of high-mannose and Complex type N-glycans.
Citation
Protein glycosylation, 39 - 48
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 15
ISSN
0930-4320
EISSN
ISBN
1560811846
3527283676
3527283676
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Attribution-NonCommercial-ShareAlike 4.0 International