Loading...
STRUCTURE-FUNCTION RELATIONSHIP IN PARATHYROID HORMONE
Wingender, Edgar Bercz, G. Hellstern, H. Schlüter, K.-D. Mayer, H.
Wingender, Edgar
Bercz, G.
Hellstern, H.
Schlüter, K.-D.
Mayer, H.
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
1989
Submitted date
2023-11-03
Files
Loading...
PDF
Adobe PDF, 5.07 MB
Other Titles
Abstract
Parathyroid hormone (PTH) is the main regulator of calcium homeostasis and controls resorption as well as formation of bone. We assayed overlapping PTH fragments for their capability to stimulate either adenylate cyclase activity or DNA synthesis in in appropriate renal or skeletal cell systems. From these studies we concluded that PTH-induced enhancement of cellular cAMP levels and of cell proliferation are exerted by different functional domains of the hormone molecule. Their conincidence with structural domains will be discussed. After establishment of an efficient expression system for human PTH in E. coli both functional and structural considerations lead to a series of hormone variants. Their biological characterization showed that individual PTH effects could selectively be affected by these mutations. Therefore, site-directed mutagenesis conceivably opens up the possibility to select for either anabolic or catabolic in vivo activities of PTH. Functional design of a potent inducer-of bone growth might be of considerable clinical interest.
Citation
Advances in protein design, 167 - 176
Affiliation
Journal
DOI
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 12
ISSN
0930-4320
EISSN
ISBN
0895739534
3527280243
3527280243
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International