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PREPARATION OF A NADH OXIDASE FOR BIOSENSOR APPLICATIONS
Kondruweit, S. Erdmann, Helmut Park, H.-J. Reiser, C. O. A. Sprinzi, M. Schmid, Rolf D.
Kondruweit, S.
Erdmann, Helmut
Park, H.-J.
Reiser, C. O. A.
Sprinzi, M.
Schmid, Rolf D.
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Issue Date
1992
Submitted date
2024-04-17
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Abstract
A NADHoxidase from Thermus thermophilus HB8 was purified to homogeneity by a procedure that is easy to scale up. The hydrogen peroxide forming NADH oxidase was found to be a monomerof 26 kD by SDSPAGE, oxidation of NADH (NADPH) occuredin the presence of O, and either FMN or FAD. These cofactors also enhancedthe stability of the enzyme towards higher temperatures and extreme pH. The properties of the NADH oxidase are discussed in respectofits applicability in biosensor techniques.
Citation
Biosensors : fundamentals, technologies and applications, 245 - 250
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
3527284370
1560812206
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International