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Sialylation Is Dispensable for Early Murine Embryonic Development in Vitro.
Abeln, Markus Borst, Kristina M Cajic, Samanta Thiesler, Hauke Kats, Elina Albers, Iris Kuhn, Maike Kaever, Volkhard Rapp, Erdmann Münster-Kühnel, Anja
Abeln, Markus
Borst, Kristina M
Cajic, Samanta
Thiesler, Hauke
Kats, Elina
Albers, Iris
Kuhn, Maike
Kaever, Volkhard
Rapp, Erdmann
Münster-Kühnel, Anja
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2017-07-04
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The negatively charged nonulose sialic acid (Sia) is essential for murine development in vivo. In order to elucidate the impact of sialylation on differentiation processes in the absence of maternal influences, we generated mouse embryonic stem cell (mESC) lines that lack CMP-Sia synthetase (CMAS) and thereby the ability to activate Sia to CMP-Sia. Loss of CMAS activity resulted in an asialo cell surface accompanied by an increase in glycoconjugates with terminal galactosyl and oligo-LacNAc residues, as well as intracellular accumulation of free Sia. Remarkably, these changes did not impact intracellular metabolites or the morphology and transcriptome of pluripotent mESC lines. Moreover, the capacity of Cmas
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1439-7633
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Attribution-NonCommercial-ShareAlike 3.0 United States