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Neutral Lipopolyplexes for In Vivo Delivery of Conventional and Replicative RNA Vaccine.

Perche, Federico
; 
Clemençon, Rudy
; ; 
Ebensen, Thomas
; 
Guzmán, Carlos A
; 
Pichon, Chantal
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Issue Date
2019-09-06
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Abstract
Nucleic acid vaccination relies on injecting DNA or RNA coding antigen(s) to induce a protective immune response. RNA vaccination is being increasingly used in preclinical and clinical studies. However, few delivery systems have been reported for in vivo delivery of RNA of different sizes. Using a tripartite formulation with RNA, cationic polymer, and anionic liposomes, we were able to encapsulate RNA into neutral lipopolyplexes (LPPs). LPPs were stable in vitro and successfully delivered conventional RNA and replicative RNA to dendritic cells in cellulo. Their injection led to reporter gene expression in mice. Finally, administration of LPP-Replicon RNA (RepRNA) led to an adaptive immune response against the antigen coded by the RepRNA. Accordingly, LPPs may represent a universal formulation for RNA delivery.
Citation
- Mol Ther Nucleic Acids. 2019 Sep 6;17:767-775. doi: 10.1016/j.omtn.2019.07.014. Epub 2019 Jul 30.
Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
Publisher
Elsevier (Cell Press)
Journal
Molecular Therapy - Nucleic Acids
URI
http://hdl.handle.net/10033/621939
DOI
10.1016/j.omtn.2019.07.014
PubMed ID
31446119
PubMed Central ID
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Article
Language
en
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ISSN
2162-2531
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Attribution-NonCommercial-ShareAlike 4.0 International
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publications of the research group vaccinology and applied microbiology (VAC)