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Establishment of an induced memory response in Pseudomonas aeruginosa during infection of a eukaryotic host.

Kordes, Adrian
; 
Grahl, Nora
; 
Koska, Michal
; 
Preusse, Matthias
; 
Arce-Rodriguez, Alejandro
; ; 
Kaever, Volkhard
; 
Häussler, Susanne
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Issue Date
2019-08-01
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Abstract
In a given habitat, bacterial cells often experience recurrent exposures to the same environmental stimulus. The ability to memorize the past event and to adjust current behaviors can lead to efficient adaptation to the recurring stimulus. Here we demonstrate that the versatile bacterium Pseudomonas aeruginosa adopts a virulence phenotype after serial passage in the invertebrate model host Galleria mellonella. The virulence phenotype was not linked to the acquisition of genetic variations and was sustained for several generations, despite cultivation of the ex vivo virulence-adapted P. aeruginosa cells under rich medium conditions in vitro. Transcriptional reprogramming seemed to be induced by a host-specific food source, as reprogramming was also observed upon cultivation of P. aeruginosa in rich medium supplemented with polyunsaturated long-chain fatty acids. The establishment of induced memory responses adds a time dimension and seems to fill the gap between long-term evolutionary genotypic adaptation and short-term induced individual responses. Efforts to unravel the fundamental mechanisms that underlie the carry-over effect to induce such memory responses will continue to be of importance as hysteretic behavior can serve survival of bacterial populations in changing and challenging habitats.
Citation
ISME J. 2019 Aug;13(8):2018-2030. doi: 10.1038/s41396-019-0412-1. Epub 2019 Apr 5.
Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
Publisher
Springer-Nature
Journal
ISME
URI
http://hdl.handle.net/10033/621896
DOI
10.1038/s41396-019-0412-1
PubMed ID
30952997
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en
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1751-7370
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openAccess
Attribution-NonCommercial-ShareAlike 4.0 International
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publications of the research group of molecular bacteriology(MOBA)
publications of the research group chemical microbiology (CMIK)