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FIRST STUDIES ON THE DEVELOPMENT OF A BIOSENSOR FOR TRIGLYCERIDES
Kallabis, B. Cammann, Karl Spener, F.
Kallabis, B.
Cammann, Karl
Spener, F.
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Issue Date
1992
Submitted date
2024-04-10
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Abstract
A new method for triglyceride analysis is described. It is based on the fluorescence measurement of NADH, which is obtained in a coupled enzymic Process, i.e., hydrolysis of triglycerides by regiounspecific lipase and subsequent oxidation of glycerol by NAD+ dependent glycerol dehydrogenase. The emerging fluorescence signal at 465 nm following excitation at 365 nm could be monitored also in turbic solutions, thus time consuming pretreatments such as extractions become obsolete. The method was also applied in a FIA-type arrangement.
Citation
Biosensors : fundamentals, technologies and applications, 71 - 74
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Type
Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
3527284370
1560812206
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International