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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).
Cossarizza, Andrea Chang, Hyun-Dong Radbruch, Andreas Acs, Andreas Adam, Dieter Adam-Klages, Sabine Agace, William W Aghaeepour, Nima Akdis, Mübeccel Allez, Matthieu
Cossarizza, Andrea
Chang, Hyun-Dong
Radbruch, Andreas
Acs, Andreas
Adam, Dieter
Adam-Klages, Sabine
Agace, William W
Aghaeepour, Nima
Akdis, Mübeccel
Allez, Matthieu
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2019-10-01
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Abstract
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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Eur J Immunol. 2019 Oct;49(10):1457-1973. doi: 10.1002/eji.201970107.
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en
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1521-4141
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Attribution-NonCommercial-ShareAlike 4.0 International