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Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol.

Beckers, Veronique
; 
Poblete-Castro, Ignacio
; 
Tomasch, Jürgen
; 
Wittmann, Christoph
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2016
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Abstract
Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, growth kinetics and stoichiometry, to gain a systematic understanding of the underlying metabolic and regulatory network.
Citation
Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol. 2016, 15 (1):73 Microb. Cell Fact.
Affiliation
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
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Journal
Microbial cell factories
URI
http://hdl.handle.net/10033/612003
DOI
10.1186/s12934-016-0470-2
PubMed ID
27142075
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Article
Language
en
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ISSN
1475-2859
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publications of the research group microbial communication (KOM)