Loading...
A high-resolution transcriptome map identifies small RNA regulation of metabolism in the gut microbe Bacteroides thetaiotaomicron.
Ryan, Daniel Jenniches, Laura Reichardt, Sarah Barquist, Lars Westermann, Alexander J
Ryan, Daniel
Jenniches, Laura
Reichardt, Sarah
Barquist, Lars
Westermann, Alexander J
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2020-07-16
Submitted date
Files
Loading...
Open Access publication
Adobe PDF, 2.7 MB
Other Titles
Abstract
Bacteria of the genus Bacteroides are common members of the human intestinal microbiota and important degraders of polysaccharides in the gut. Among them, the species Bacteroides thetaiotaomicron has emerged as the model organism for functional microbiota research. Here, we use differential RNA sequencing (dRNA-seq) to generate a single-nucleotide resolution transcriptome map of B. thetaiotaomicron grown under defined laboratory conditions. An online browser, called 'Theta-Base' ( www.helmholtz-hiri.de/en/datasets/bacteroides ), is launched to interrogate the obtained gene expression data and annotations of ~4500 transcription start sites, untranslated regions, operon structures, and 269 noncoding RNA elements. Among the latter is GibS, a conserved, 145 nt-long small RNA that is highly expressed in the presence of N-acetyl-D-glucosamine as sole carbon source. We use computational predictions and experimental data to determine the secondary structure of GibS and identify its target genes. Our results indicate that sensing of N-acetyl-D-glucosamine induces GibS expression, which in turn modifies the transcript levels of metabolic enzymes.
Citation
Nat Commun. 2020 Jul 16;11(1):3557. doi: 10.1038/s41467-020-17348-5.
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
EISSN
2041-1723
ISBN
ISMN
Gov't Doc #
Sponsors
License
Attribution 4.0 International