Loading...
A global data-driven census of Salmonella small proteins and their potential functions in bacterial virulence
Venturini, Elisa Svensson, Sarah L Maaß, Sandra Gelhausen, Rick Eggenhofer, Florian Li, Lei Cain, Amy K Parkhill, Julian Becher, Dörte Backofen, Rolf
Venturini, Elisa
Svensson, Sarah L
Maaß, Sandra
Gelhausen, Rick
Eggenhofer, Florian
Li, Lei
Cain, Amy K
Parkhill, Julian
Becher, Dörte
Backofen, Rolf
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2020-10-17
Submitted date
Files
Loading...
Open Access publication
Adobe PDF, 3.26 MB
Other Titles
Abstract
Small proteins are an emerging class of gene products with diverse roles in bacterial physiology. However, a full understanding of their importance has been hampered by insufficient genome annotations and a lack of comprehensive characterization in microbes other than Escherichia coli. We have taken an integrative approach to accelerate the discovery of small proteins and their putative virulence-associated functions in Salmonella Typhimurium. We merged the annotated small proteome of Salmonella with new small proteins predicted with in silico and experimental approaches. We then exploited existing and newly generated global datasets that provide information on small open reading frame expression during infection of epithelial cells (dual RNA-seq), contribution to bacterial fitness inside macrophages (Transposon-directed insertion sequencing), and potential engagement in molecular interactions (Grad-seq). This integrative approach suggested a new role for the small protein MgrB beyond its known function in regulating PhoQ. We demonstrate a virulence and motility defect of a Salmonella ΔmgrB mutant and reveal an effect of MgrB in regulating the Salmonella transcriptome and proteome under infection-relevant conditions. Our study highlights the power of interpreting available ‘omics’ datasets with a focus on small proteins, and may serve as a blueprint for a data integration-based survey of small proteins in diverse bacteria.
Citation
MicroLife(2020) .advance publication; DOI: 10.1093/femsml/uqaa002 .
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
EISSN
2633-6693
ISBN
ISMN
Gov't Doc #
Sponsors
Australian Research Council
License
Attribution 4.0 International