Loading...
Generation of human antigen-specific monoclonal IgM antibodies using vaccinated "human immune system" mice.
Becker, Pablo D Legrand, Nicolas van Geelen, Caroline M M Noerder, Miriam Huntington, Nicholas D Lim, Annick Yasuda, Etsuko Diehl, Sean A Scheeren, Ferenc A Ott, Michael
Becker, Pablo D
Legrand, Nicolas
van Geelen, Caroline M M
Noerder, Miriam
Huntington, Nicholas D
Lim, Annick
Yasuda, Etsuko
Diehl, Sean A
Scheeren, Ferenc A
Ott, Michael
Files
Loading...
Open Access publication
Adobe PDF, 1.49 MB
Other Titles
Abstract
Passive transfer of antibodies not only provides immediate short-term protection against disease, but also can be exploited as a therapeutic tool. However, the 'humanization' of murine monoclonal antibodies (mAbs) is a time-consuming and expensive process that has the inherent drawback of potentially altering antigenic specificity and/or affinity. The immortalization of human B cells represents an alternative for obtaining human mAbs, but relies on the availability of biological samples from vaccinated individuals or convalescent patients. In this work we describe a novel approach to generate fully human mAbs by combining a humanized mouse model with a new B cell immortalization technique.
Citation
Generation of human antigen-specific monoclonal IgM antibodies using vaccinated "human immune system" mice. 2010, 5 (10) PLoS ONE
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1932-6203