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Activity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria.

Kaufmann, Katrin
; 
Simmons, Luke
; 
Herrmann, Jennifer
; 
Schwär, Gertrud
; 
Luniak, Nora
; 
Müller, Rolf
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Issue Date
2013-01
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Abstract
Using an in vitro cell-based assay in a flow-design, we have applied activity-guided screening to search for new bioactive compounds isolated from microorganisms. A first assay employs the stable expression of nuclear factor kappa B (NF-κB) while a second assay utilizes the glucocorticoid receptor (GR) coupled to green fluorescent protein. A specialized assay was implemented for both the translocation of NF-κB and to inhibit the translocation of cytokine-mediated NF-κB. In addition, we developed in a wide palette of cell lines used for a highly specialized GR-translocation assay to detect anti-inflammatory effects. This approach demonstrates the straight-forward combination of cell-based assays arranged with an automated fluorescence microscope. This allows for the direct sorting of extracts which are acting in a pharmaceutically interesting way. Initial results using this technique have led to the detection of new anti-inflammatory steroids from bacterial crude extracts.
Citation
Activity-guided screening of bioactive natural compounds implementing a new glucocorticoid-receptor-translocation assay and detection of new anti-inflammatory steroids from bacteria. 2013, 35 (1):11-20 Biotechnol. Lett.
Affiliation
Department of Pharmaceutical Biotechnology, Saarland University, Saarbrücken, Germany. kaufm@uni-trier.de
Publisher
Journal
Biotechnology letters
URI
http://hdl.handle.net/10033/270921
DOI
10.1007/s10529-012-1042-0
PubMed ID
22983717
PubMed Central ID
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Article
Language
en
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ISSN
1573-6776
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Archived with thanks to Biotechnology letters
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publications of the research group of microbial natural substances([HIPS]MINS)