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DEVELOPMENT OF AN AGAR-MEDIUM (ESCULIN CELLOBIOSE AGAR) FOR THE DETECTION AND ENUMERATION OF LACTOBACILLUS ACIDOPHILUS
Hunger, W.
Hunger, W.
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Issue Date
1989
Submitted date
2023-10-11
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Abstract
An agar-medium (Esculin cellobiose agar) is presented which enables the detection and quantitative determination of Lactobacillus acidophilus in dairy products, especially in fermented milk products. For this purpose the ability of L.acidophilus to grow at 40 °C, to ferment cellobiose, and to hydrolyse esculin to esculetine is used. Esculetine forms in combination with Fe (III) ions and olive-green to black complex. For a further differentiation the addition of chlorophenolred was useful. In total 60 strains of lactobacilli, streptococci, and bifidobacteria from 22 species were examined regarding their growth on EC-agar. Only L.acidophilus, L.casei-rhamnosus and L.plantarum could not be differentiated distinctly.
Citation
Enzyme in der Lebensmitteltechnologie, 1989, 281 - 284
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF Monographien, Band 11
ISSN
0930-4320
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ISBN
3-527-27877-X
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Attribution-NonCommercial-ShareAlike 4.0 International