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MONITORING AND CONTROL OF BIOTECHNOLOGICAL PRODUCTION PROCESSES BY BIO-FET-FIA-SENSORS
Brand, U. Brandes, L. Koch, V. Kullik, T. Reinhardt, B. Rüther, F. Scheper, Thomas Schügerl, K. Wang, S. Wu, X.
Brand, U.
Brandes, L.
Koch, V.
Kullik, T.
Reinhardt, B.
Rüther, F.
Scheper, Thomas
Schügerl, K.
Wang, S.
Wu, X.
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Issue Date
1992
Submitted date
2024-04-30
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Abstract
Single and multisensor Field Effect Transistors FET with a pH-sensitive Si/SiO2/SizN4/TazOs-gate and reference electrode (for single semsor) were developed and used for manufacturing the following BIO-FETs: for glucose analysis: Glucose Oxidase-FETs (GOD-FET), for penicillin G and V analysis: Penicillin G Amidase- and Penicillinase-FETs, for urea analysis: Urease-FET, and for cephalosporin C analysis: Cephalosporinase-FET. The GOD-FETswereintegrated into the FIA-system of Eppendorf (EVA) and used for monitoring the glucose concentration in microbial cultivation and production processes with recombinant_Escherichia coli K12 MF, recombinant Escherichia coli JM103, Saccharomyces cerevisiae H620, and Candida boidinii. Urease-FET-FIA was used to monitor the urea concentration in a simulatedcultivation of Cephalosporium acremonium and UREASE-FET-FIA and GOD-FET-FIA for the monitoring of urea and glucose concentrations in simulated Saccharomyces cerevisiae cultivations.
Citation
Biosensors : fundamentals, technologies and applications, 311 - 319
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
EISSN
ISBN
1560812206
3527284370
3527284370
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Attribution-NonCommercial-ShareAlike 4.0 International