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Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation.

Reck, Michael
; 
Tomasch, Jürgen
; 
Deng, Zhiluo
; 
Jarek, Michael
; 
Husemann, Peter
; 
Wagner-Döbler, Irene
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2015
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Abstract
The complex microbiome of the gut has an enormous impact on human health. Analysis of the transcriptional activity of microorganisms through mRNA sequencing (metatranscriptomics) opens a completely new window into their activity in vivo, but it is highly challenging due to numerous technical and bioinformatical obstacles. Here we present an optimized pipeline for extraction of high quality mRNA from stool samples.
Citation
Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation. 2015, 16:494 BMC Genomics
Affiliation
Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Publisher
Journal
BMC genomics
URI
http://hdl.handle.net/10033/564203
DOI
10.1186/s12864-015-1694-y
PubMed ID
26140923
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Article
Language
en
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ISSN
1471-2164
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publications of the research group microbial communication (KOM)