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Aurantimycin resistance genes contribute to survival of Listeria monocytogenes during life in the environment.
Hauf, Samuel Herrmann, Jennifer Miethke, Marcus Gibhardt, Johannes Commichau, Fabian M Fuchs, Stephan Halbedel, Sven
Hauf, Samuel
Herrmann, Jennifer
Miethke, Marcus
Gibhardt, Johannes
Commichau, Fabian M
Fuchs, Stephan
Halbedel, Sven
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2019-01-15
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Bacteria can cope with toxic compounds such as antibiotics by inducing genes for their detoxification. A common detoxification strategy is compound excretion by ATP-binding cassette (ABC) transporters, which are synthesized upon compound contact. We previously identified the multi drug resistance ABC transporter LieAB in Listeria monocytogenes, a Gram-positive bacterium that occurs ubiquitously in the environment, but also causes severe infections in humans upon ingestion. Expression of the lieAB genes is strongly induced in cells lacking the PadR-type transcriptional repressor LftR, but compounds leading to relief of this repression in wild type cells were not known. Using RNA-Seq and promoter-lacZ fusions we demonstrate highly specific repression of the lieAB and lftRS promoters through LftR. Screening of a natural compound library yielded the depsipeptide aurantimycin A - synthesized by the soil-dwelling Streptomyces aurantiacus - as the first known naturally occurring inducer of lieAB expression. Genetic and phenotypic experiments concordantly show that aurantimycin A is a substrate of the LieAB transporter and thus, lftRS and lieAB represent the first known genetic module conferring and regulating aurantimycin A resistance. Collectively, these genes may support the survival of L. monocytogenes when it comes into contact with antibiotic-producing bacteria in the soil. This article is protected by copyright. All rights reserved.
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en
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1365-2958
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Attribution-NonCommercial-ShareAlike 4.0 International