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Enzymes and Antibodies for Biosensors - Studies at the GBF
Schmid, Rolf D.
Schmid, Rolf D.
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Issue Date
1992
Submitted date
2024-04-17
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Abstract
Enzymes and antibodies are key components of most biosensors and have also found wide application in instrumental analysis, e.g. in flow injection analysis [1, 2]. Among the enzymes studied in enzyme sensors, oxidases are of particular importance since they consume oxygen and form hydrogenperoxidetwo species which can easily be monitored by electrochemical or photometric transducers. While many oxidases with a wide range ofsubstrate specificity are commercially available, we have set out to further expand this range by the methods of a) screening of suitable microbial enzymes,and b) by applying the techniques of protein engineering. So far, we have successfully screened a microbial amino acid oxidase, a microbial xanthine oxidase and a microbial acyl-CoA oxidase with unusual substrate specificity. In another part of our work focussed on the "protein design" of biosensor-related proteins, we have solved the structures of two glucose oxidases, purified to homogeneity a thermophilic, hydrogen peroxide forming NADH oxidase, and purified and crystallized a bacterial FMN-dependentluciferase. In the field of immunoreagents for biosensors, we have set out to solve the structure of a pesticide-specific monoclonal antibody. The status and the focus of these investigations will be discussed.
Citation
Biosensors : fundamentals, technologies and applications, 229 - 234
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
3527284370
1560812206
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International