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miR-20a inhibits TCR-mediated signaling and cytokine production in human naïve CD4+ T cells.
Reddycherla, Amarendra V Meinert, Ines Reinhold, Annegret Reinhold, Dirk Schraven, Burkhart Simeoni, Luca
Reddycherla, Amarendra V
Meinert, Ines
Reinhold, Annegret
Reinhold, Dirk
Schraven, Burkhart
Simeoni, Luca
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Abstract
Upon TCR stimulation by peptide-MHC complexes, CD4+ T cells undergo activation and proliferation. This process will ultimately culminate in T-cell differentiation and the acquisition of effector functions. The production of specific cytokines by differentiated CD4+ T cells is crucial for the generation of the appropriate immune response. Altered CD4+ T-cell activation and cytokine production result in chronic inflammatory conditions and autoimmune disorders. miRNAs have been shown to be important regulators of T-cell biology. In this study, we have focused our investigation on miR-20a, a member of the miR-17-92 cluster, whose expression is decreased in patients suffering from multiple sclerosis. We have found that miR-20a is rapidly induced upon TCR-triggering in primary human naïve CD4+ T cells and that its transcription is regulated in a Erk-, NF-κB-, and Ca++-dependent manner. We have further shown that overexpression of miR-20a inhibits TCR-mediated signaling but not the proliferation of primary human naïve CD4+ T cells. However, miR-20a overexpression strongly suppresses IL-10 secretion and moderately decreases IL-2, IL-6 and IL8 production, which are crucial regulators of inflammatory responses. Our study suggests that miR-20a is a new player in the regulation of TCR signaling strength and cytokine production.
Citation
miR-20a inhibits TCR-mediated signaling and cytokine production in human naïve CD4+ T cells. 2015, 10 (4):e0125311 PLoS ONE
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Article
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en
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1932-6203