Loading...
SENSITIVE DETECTION OF PROTEIN ADSORPTION TO SUPPORTEDLIPID BILAYERS BY CAPACITANCE MEASUREMENTS
Stelzle, Martin Sackmann, Erich
Stelzle, Martin
Sackmann, Erich
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
1989
Submitted date
2024-01-16
Files
Loading...
PDF
Adobe PDF, 3.68 MB
Other Titles
Abstract
Wereport experiments on the sensitive detection of protein adsorption to lipid bilayers deposited onto chromium electrodes on glass substrates by capacitance measurements. The sensitivity of the present type of sensor (better than 3A average protein layer thickness) is at least equivalent to that of ellipsometry. A high specific resistance of the supported bilayer of (1-5)-10%cm® is achieved by deposition of a tightly packed (crystalline) cadmium arachidate monolayer in contact with the substrate, whereas the outer monolayer can be more loosely packed (fluid phase or state of fluid-solid coexistence) which is essential for the incorporation of receptors. In the present work, charged lipids are incorporated as nonspecific receptors for polylysine and cytochrome c. Furthermore, the capacitance measurements provide a very sensitive test for the tightness and long-time stability of supported bilayers.
Citation
Biosensors : applications in medicine, environmental protection and process control, 339 - 346
DOI
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
EISSN
ISBN
3527280324
0895739550
0895739550
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International