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Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane.
Zamprogno, Pauline Wüthrich, Simon Achenbach, Sven Thoma, Giuditta Stucki, Janick D Hobi, Nina Schneider-Daum, Nicole Lehr, Claus-Michael Huwer, Hanno Geiser, Thomas
Zamprogno, Pauline
Wüthrich, Simon
Achenbach, Sven
Thoma, Giuditta
Stucki, Janick D
Hobi, Nina
Schneider-Daum, Nicole
Lehr, Claus-Michael
Huwer, Hanno
Geiser, Thomas
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2021-02-05
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Abstract
The air-blood barrier with its complex architecture and dynamic environment is difficult to mimic in vitro. Lung-on-a-chips enable mimicking the breathing movements using a thin, stretchable PDMS membrane. However, they fail to reproduce the characteristic alveoli network as well as the biochemical and physical properties of the alveolar basal membrane. Here, we present a lung-on-a-chip, based on a biological, stretchable and biodegradable membrane made of collagen and elastin, that emulates an array of tiny alveoli with in vivo-like dimensions. This membrane outperforms PDMS in many ways: it does not absorb rhodamine-B, is biodegradable, is created by a simple method, and can easily be tuned to modify its thickness, composition and stiffness. The air-blood barrier is reconstituted using primary lung alveolar epithelial cells from patients and primary lung endothelial cells. Typical alveolar epithelial cell markers are expressed, while the barrier properties are preserved for up to 3 weeks.
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Commun Biol. 2021 Feb 5;4(1):168. doi: 10.1038/s42003-021-01695-0.
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en
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2399-3642
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Attribution 4.0 International