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X-RAY STRUCTURE ANALYSIS OF GEOTRICHUM CANDIDUM LIPASE AT 2.8 A RESOLUTION
Sugihara, Akio Hata, Yasuo Shimada, Yuji Tominaga, Yoshio Katsube, Yukiteru
Sugihara, Akio
Hata, Yasuo
Shimada, Yuji
Tominaga, Yoshio
Katsube, Yukiteru
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Issue Date
1991
Submitted date
2024-03-13
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Abstract
Geotrichum candidum (ATCC 34614) produces extracellular lipase upon submerged cultivation. With conventional purification techniques, the enzyme was easily purified to apparent homogeneity, as judged from disc- PAGE, isoelectric focusing and ultracentrifiugation. It was easily crystallized by concentration, and low resolution X-ray analysis was done on the crystals. However, we have quite recently found that the apparently pure lipase was a mixture of two isozymes, both composed of 544 amino acids and 6.5% carbohydrate. The two isozymes were separated from each other by hydrophobic interaction chromatography. An X-ray analysis at 2.8 A resolution has been undertaken onthe main fraction. The main chain was tentatively followed. The whole enzyme molecule consists of a single domain of 50 x 50 x 70 A, and contains 9 a-helices and 2 g-sheets.
Citation
Lipases : structure, mechanism and genetic engineering, 47 - 54
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International