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Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans.
Wohlschlager, Therese Titz, Alexander Künzler, Markus Varrot, Annabelle
Wohlschlager, Therese
Titz, Alexander
Künzler, Markus
Varrot, Annabelle
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2020-04-19
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Abstract
Tectonins are conserved defense proteins of innate immune systems featuring a β-propeller fold. Tectonin 2 from Laccaria bicolor, Lb-Tec2, is the first fungal representative of the tectonin superfamily that has been described. In-depth characterization revealed a specificity for O-methylated glycans and identified a unique sequence motif and binding site architecture underlying this unusual specificity. This chapter provides information on how to produce and purify recombinant Lb-Tec2, characterize its interaction with O-methylated glycans and demonstrate its biological function.
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Methods Mol Biol. 2020;2132:669-682. doi: 10.1007/978-1-0716-0430-4_58.
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Article
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en
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1940-6029
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Attribution 4.0 International