Loading...
DEVELOPMENT OF HIGH SENSITIVE AMPEROMETRIC ENZYME ELECTRODES
Kulys, J. J.
Kulys, J. J.
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
1987
Submitted date
2023-07-19
Files
Loading...
PDF
Adobe PDF, 5.65 MB
Other Titles
Abstract
High sensitive amperometric enzyme electrodes based on chemical emplification in biocatalytic membranes or biocatalytic accumulation of oxidation-reduction equivalents on chemically modified electrodes have been designed. Chemical amplification via cyclic substrate conversion in biocatalytic membranes proceeds merely at high diffusion moduli values. The amplification degree depends on the catalytic activity of membranes and their thickness as well. The response of the electrodes with cyclic substrate conversion is slow. High sensitive determination of aromatic amines was carried out with the use of enzyme electrodes in the biocatalytic layer of which the substrate-substrate activation of peroxidase catalysis occurs. The activation in the bienzyme glucose oxidase/peroxidase system is observed at high glucose concentrations. Biocatalytic product accumulation, accomplished through the apoenzyme and cofactor interaction, enables to determine a substance in concentration relative to the dissociation constant of choloenzyme. The accumulation of oxidation-reduction equivalents on chemically modified electrodes with their subsequent electrochemical conversion makes the basis for stripping analysis, ascertaining the determination of low metabolite concentrations.
Citation
Biosensors International Workshop 1987, 51 - 60
DOI
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF Monographs, Volume 10
ISSN
0930-4320
EISSN
ISBN
0-89573-683-7
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International