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CRYSTALLIZATION OF GASTRIC LIPASES
Abergel, Chantal Fontecilla, Juan Cambillau, Christian
Abergel, Chantal
Fontecilla, Juan
Cambillau, Christian
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Issue Date
1991
Submitted date
2024-03-06
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Abstract
Gastric lipases are pH resistant enzymes with a molecular weight of ca 50,000, including 15% sugars. Every glyco-variant from different species have been purified by ief, and submitted to crystallization, using parameters found by an incomplete factorial analysis method. All attempts were successful, but most crystals were unsuitable to X-ray studies due to huge cell dimensions. Dog gastric lipase crystals, the most suitable for X-ray study (pH 6.8, 12% PEG 6000, 20°C; P2242 :182A, 211A, 98 A), contain ca 8 molecules in the asymetric unit , and therefore diffract to only 4A on lab sources.
Citation
Lipases : structure, mechanism and genetic engineering, 27 - 30
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International