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The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate.
Lu, Xin Sun, Jibin Nimtz, Manfred Wissing, Josef Zeng, An-Ping Rinas, Ursula
Lu, Xin
Sun, Jibin
Nimtz, Manfred
Wissing, Josef
Zeng, An-Ping
Rinas, Ursula
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Abstract
The filamentous fungus Aspergillus niger is well-known as a producer of primary metabolites and extracellular proteins. For example, glucoamylase is the most efficiently secreted protein of Aspergillus niger, thus the homologous glucoamylase (glaA) promoter as well as the glaA signal sequence are widely used for heterologous protein production. Xylose is known to strongly repress glaA expression while maltose is a potent inducer of glaA promoter controlled genes. For a more profound understanding of A. niger physiology, a comprehensive analysis of the intra- and extracellular proteome of Aspergillus niger AB1.13 growing on defined medium with xylose or maltose as carbon substrate was carried out using 2-D gel electrophoresis/Maldi-ToF and nano-HPLC MS/MS.
Citation
The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate. 2010, 9:23 Microb. Cell Fact.
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Article
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en
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Series/Report no.
ISSN
1475-2859