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Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro.
Schwagerus, Elena Sladek, Svenja Buckley, Stephen T Armas-Capote, Natalia Alvarez de la Rosa, Diego Harvey, Brian J Fischer, Horst Illek, Beate Huwer, Hanno Schneider-Daum, Nicole
Schwagerus, Elena
Sladek, Svenja
Buckley, Stephen T
Armas-Capote, Natalia
Alvarez de la Rosa, Diego
Harvey, Brian J
Fischer, Horst
Illek, Beate
Huwer, Hanno
Schneider-Daum, Nicole
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2015-11
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Abstract
Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (I SC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in I SC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption.
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Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro. 2015, 467 (11):2257-73 Pflugers Arch.
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en
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1432-2013