Loading...
Depletion of regulatory T cells augments a vaccine-induced T effector cell response against the liver-stage of malaria but fails to increase memory.
Espinoza Mora, Maria del Rosario Steeg, Christiane Tartz, Susanne Heussler, Volker Sparwasser, Tim Link, Andreas Fleischer, Bernhard Jacobs, Thomas
Espinoza Mora, Maria del Rosario
Steeg, Christiane
Tartz, Susanne
Heussler, Volker
Sparwasser, Tim
Link, Andreas
Fleischer, Bernhard
Jacobs, Thomas
Files
Loading...
Open Access publication
Adobe PDF, 1.27 MB
Other Titles
Abstract
Regulatory T cells (T(reg)) have been shown to restrict vaccine-induced T cell responses in different experimental models. In these studies CD4(+)CD25(+) T(reg) were depleted using monoclonal antibodies against CD25, which might also interfere with CD25 on non-regulatory T cell populations and would have no effect on Foxp3(+)CD25(-) T(reg). To obtain more insights in the specific function of T(reg) during vaccination we used mice that are transgenic for a bacterial artificial chromosome expressing a diphtheria toxin (DT) receptor-eGFP fusion protein under the control of the foxp3 gene locus (depletion of regulatory T cell mice; DEREG). As an experimental vaccine-carrier recombinant Bordetella adenylate cyclase toxoid fused with a MHC-class I-restricted epitope of the circumsporozoite protein (ACT-CSP) of Plasmodium berghei (Pb) was used. ACT-CSP was shown by us previously to introduce the CD8+ epitope of Pb-CSP into the MHC class I presentation pathway of professional antigen-presenting cells (APC). Using this system we demonstrate here that the number of CSP-specific T cells increases when T(reg) are depleted during prime but also during boost immunization. Importantly, despite this increase of T effector cells no difference in the number of antigen-specific memory cells was observed.
Citation
Depletion of regulatory T cells augments a vaccine-induced T effector cell response against the liver-stage of malaria but fails to increase memory. 2014, 9 (8):e104627 PLoS ONE
Affiliation
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1932-6203
EISSN
ISBN
ISMN
Gov't Doc #
Sponsors
License
Archived with thanks to PloS one