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Decreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b.
Glaesener, Stephanie Jaenke, Christine Habener, Anika Hagendorff, Petra Witzlau, Katrin Imelmann, Esther Krueger, Andreas Meyer-Bahlburg, Almut
Glaesener, Stephanie
Jaenke, Christine
Habener, Anika
Hagendorff, Petra
Witzlau, Katrin
Imelmann, Esther
Krueger, Andreas
Meyer-Bahlburg, Almut
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Abstract
The increased susceptibility to infections of neonates is caused by an immaturity of the immune system as a result of both qualitative and quantitative differences between neonatal and adult immune cells. With respect to B cells, neonatal antibody responses are known to be decreased. Accountable for this is an altered composition of the neonatal B cell compartment towards more immature B cells. However, it remains unclear whether the functionality of individual neonatal B cell subsets is altered as well. In the current study we therefore compared phenotypical and functional characteristics of corresponding neonatal and adult B cell subpopulations. No phenotypic differences could be identified with the exception of higher IgM expression in neonatal B cells. Functional analysis revealed differences in proliferation, survival, and B cell receptor signaling. Most importantly, neonatal B cells showed severely impaired class-switch recombination (CSR) to IgG and IgA. This was associated with increased expression of miR-181b in neonatal B cells. Deficiency of miR-181b resulted in increased CSR. With this, our results highlight intrinsic differences that contribute to weaker B cell antibody responses in newborns.
Citation
Decreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b. 2018, 13 (2):e0192230 PLoS ONE
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Article
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en
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Series/Report no.
ISSN
1932-6203