Loading...
SIMULTANEOUS FLOW INJECTION ANALYSIS OF L-LACTATE AND L-MALATE IN WINE BASED ON THE USE OF ENZYMES
Ukeda, Hiroyuki Yoshioka, Shuichi Matsumoto, Kiyoshi Osajima, Yutaka
Ukeda, Hiroyuki
Yoshioka, Shuichi
Matsumoto, Kiyoshi
Osajima, Yutaka
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
1992
Submitted date
2024-04-17
Files
Loading...
PDF
Adobe PDF, 2.2 MB
Other Titles
Abstract
The simultaneous determination of L-malate and L-lactate, by enzymesupported FIA, was developed using two enzymereactors in parallel and a single oxygen electrode. NADH formed in the reaction of malate dehydrogenase (MDH) was regenerated to NAD with dissolved oxygen,using vitamin K3 and diaphorase (DI). L-Lactate was determined using the enzymelactate oxidase (LOD). When sample solutions were simultaneously injected into the two reactors (the MDH-Di-reactor and the LOD-reactor) with a controlled residence time, a train of two peaks corresponding to L-lactate and L-malate were seen in the FIA-gram. The peak currents were linearly related to the Lmalate and L-lactate concentration in the range 0.05-1.2 mM and 0.01-0.5 mM, respectively. The present system was applied to the determination of Llactate and L-malate in white wine. The results showed a good agreement with those obtained using a conventional method (F-Kit method), suggesting that this system may be applicable to the monitoring of malo-lactic fermentation during wine production.
Citation
Biosensors : fundamentals, technologies and applications, 225 - 228
DOI
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
EISSN
ISBN
3527284370
1560812206
1560812206
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International