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ENZYMATIC ASSAY BY FLOW INJECTION ANALYSIS WITH DETECTION BY CHEMILUMINESCENCE: DETERMINATION OF GLUCOSE, CREATININE, FREE CHOLESTEROL AND LACTIC ACID USING AN INTEGRATED FIA MICROCONDUIT
Petersson, Bo A.
Petersson, Bo A.
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Issue Date
1987
Submitted date
2023-09-06
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Abstract
A miniaturized flow injection system for the determination of D-glucose, L-lactic acid creatinine and free cholesterol is described. All substrates are degraded enzymatically by means of oxidases which, along with ancillary coenzymes (creatinine), are immobilized on controlled porosity glass and incorporated into small pvc column reactors. The hydrogen peroxide generated by the individual oxidases is determined by chemiluminescence with an alkaline reagent containing luminol and hexacyanoferrate (III). The injection valve, flow channels, enzyme reactor and light detector are integrated into a FIA microconduit. The enzyme reactors all showed little change in activity over a 3 months period of Operation and were found fully compatible with serum samples.
Citation
Biosensors International Workshop 1987, 325 - 326
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF Monographs, Volume 10
ISSN
0930-4320
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ISBN
0-89573-683-7
3-527-26801-4
3-527-26801-4
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Attribution-NonCommercial-ShareAlike 4.0 International