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Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes.
Dandekar, Prajakta Jain, Ratnesh Keil, Manuel Loretz, Brigitta Muijs, Leon Schneider, Marc Auerbach, Dagmar Jung, Gregor Lehr, Claus-Michael Wenz, Gerhard
Dandekar, Prajakta
Jain, Ratnesh
Keil, Manuel
Loretz, Brigitta
Muijs, Leon
Schneider, Marc
Auerbach, Dagmar
Jung, Gregor
Lehr, Claus-Michael
Wenz, Gerhard
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2012-12-28
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Abstract
Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest.
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Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes. 2012, 164 (3):387-93 J Control Release
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Article
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en
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1873-4995
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Archived with thanks to Journal of controlled release : official journal of the Controlled Release Society