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IL-1β Promotes Biofilms on Implants .
Gutierrez Jauregui, Rodrigo Fleige, Henrike Bubke, Anja Rohde, Manfred Weiss, Siegfried Förster, Reinhold
Gutierrez Jauregui, Rodrigo
Fleige, Henrike
Bubke, Anja
Rohde, Manfred
Weiss, Siegfried
Förster, Reinhold
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2019-01-01
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Abstract
Implant associated infections represent a serious health burden in clinics since some microorganisms are able to colonize biological surfaces or surfaces of indwelling medical devices and form biofilms. Biofilms represent communities of microorganisms attached to hydrated surfaces and enclosed in self-produced extracellular matrix. This renders them resistant to exogenous assaults like antibiotics or immune effector mechanisms. Little is known regarding the role of the immune system in the formation of biofilms during implant associated infections, largely due to the lack of suitable mouse models. Here we use colonized osmotic pumps in mice to study the interaction of an activated immune system with biofilm-forming Staphylococcus aureus encoding Gaussia luciferase. This approach permits biofilm formation on the osmotic pumps in living animals. It also allows the continuous supply of soluble immune cell activating agents, such as cytokines to study their effect on biofilm formation in vivo. Using non-invasive imaging of the bioluminescent signal emitted by the lux expressing bacteria for quantification of bacterial load in conjunction with light and electron microscopy, we observed that pump-supplied pro-inflammatory cytokine IL-1β strongly increased biofilm formation along with a massive influx of neutrophils adjacent to the biofilm-coated pumps. Thus, our data demonstrate that immune defense mechanisms can augment biofilm formation.
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Front Immunol. 2019 May 17;10:1082. doi: 10.3389/fimmu.2019.01082. eCollection 2019.
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1664-3224
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Attribution-NonCommercial-ShareAlike 4.0 International