Department of System immunology ([BRICS]SIMM)http://hdl.handle.net/10033/6206592024-02-02T18:18:09Z2024-02-02T18:18:09ZModelling collective cell motion: are on- and off-lattice models equivalent?Nava-Sedeño, Josué ManikVoß-Böhme, AnjaHatzikirou, HaralamposDeutsch, AndreasPeruani, Fernandohttp://hdl.handle.net/10033/6232432022-08-11T01:54:35Z2020-07-27T00:00:00ZModelling collective cell motion: are on- and off-lattice models equivalent?
Nava-Sedeño, Josué Manik; Voß-Böhme, Anja; Hatzikirou, Haralampos; Deutsch, Andreas; Peruani, Fernando
2020-07-27T00:00:00ZA dynamic CD2-rich compartment at the outer edge of the immunological synapse boosts and integrates signals.Demetriou, PhilipposAbu-Shah, EnasValvo, SalvatoreMcCuaig, SarahMayya, VivekaKvalvaag, AudunStarkey, ThomasKorobchevskaya, KseniyaLee, Lennard Y WFriedrich, MatthiasMann, ElizabethKutuzov, Mikhail AMorotti, MatteoWietek, NinaRada, HeatherYusuf, ShamsideenAfrose, JehanSiokis, AnastasiosMeyer-Hermann, MichaelAhmed, Ahmed AshourDepoil, DavidDustin, Michael Lhttp://hdl.handle.net/10033/6232382022-08-11T01:55:06Z2020-09-14T00:00:00ZA dynamic CD2-rich compartment at the outer edge of the immunological synapse boosts and integrates signals.
Demetriou, Philippos; Abu-Shah, Enas; Valvo, Salvatore; McCuaig, Sarah; Mayya, Viveka; Kvalvaag, Audun; Starkey, Thomas; Korobchevskaya, Kseniya; Lee, Lennard Y W; Friedrich, Matthias; Mann, Elizabeth; Kutuzov, Mikhail A; Morotti, Matteo; Wietek, Nina; Rada, Heather; Yusuf, Shamsideen; Afrose, Jehan; Siokis, Anastasios; Meyer-Hermann, Michael; Ahmed, Ahmed Ashour; Depoil, David; Dustin, Michael L
The CD2-CD58 recognition system promotes adhesion and signaling and counters exhaustion in human T cells. We found that CD2 localized to the outer edge of the mature immunological synapse, with cellular or artificial APC, in a pattern we refer to as a 'CD2 corolla'. The corolla captured engaged CD28, ICOS, CD226 and SLAM-F1 co-stimulators. The corolla amplified active phosphorylated Src-family kinases (pSFK), LAT and PLC-γ over T cell receptor (TCR) alone. CD2-CD58 interactions in the corolla boosted signaling by 77% as compared with central CD2-CD58 interactions. Engaged PD-1 invaded the CD2 corolla and buffered CD2-mediated amplification of TCR signaling. CD2 numbers and motifs in its cytoplasmic tail controlled corolla formation. CD8+ tumor-infiltrating lymphocytes displayed low expression of CD2 in the majority of people with colorectal, endometrial or ovarian cancer. CD2 downregulation may attenuate antitumor T cell responses, with implications for checkpoint immunotherapies.
2020-09-14T00:00:00ZA minimal modeling framework of radiation and immune system synergy to assist radiotherapy planning.Montaseri, GhazalAlfonso, Juan Carlos LópezHatzikirou, HaralamposMeyer-Hermann, Michaelhttp://hdl.handle.net/10033/6232262022-06-15T02:52:29Z2020-02-07T00:00:00ZA minimal modeling framework of radiation and immune system synergy to assist radiotherapy planning.
Montaseri, Ghazal; Alfonso, Juan Carlos López; Hatzikirou, Haralampos; Meyer-Hermann, Michael
2020-02-07T00:00:00ZNaive- and Memory-like CD21 B Cell Subsets Share Core Phenotypic and Signaling Characteristics in Systemic Autoimmune Disorders.Freudenhammer, MirjamVoll, Reinhard EBinder, Sebastian CKeller, BaerbelWarnatz, Klaushttp://hdl.handle.net/10033/6232232022-06-15T02:53:16Z2020-09-09T00:00:00ZNaive- and Memory-like CD21 B Cell Subsets Share Core Phenotypic and Signaling Characteristics in Systemic Autoimmune Disorders.
Freudenhammer, Mirjam; Voll, Reinhard E; Binder, Sebastian C; Keller, Baerbel; Warnatz, Klaus
An expansion of CD21low B cells has been described in a variety of diseases associated with persistent immune stimulation as in chronic infection, immunodeficiency, or autoimmunity. Different developmental stages of CD21low B cells have been highlighted in specific diseases; however, a systematic comparison of distribution, phenotype, and signaling capacity of these populations has not yet been performed to delineate the pivotal character of this unusual B cell population. Screening of more than 200 patients with autoimmune disease demonstrated that the prevalence of patients with expanded CD21low B cells varies between diseases. The expansion was frequent in patients with systemic lupus erythematosus, in which it correlated to relative B cell lymphopenia and duration of disease. Different proportions of distinct developmental stages of CD21low B cells co-occur in nearly all patients with autoimmune disease. Although in most patients, naive-like and CD27- switched memory B cells were the most prominent CD21low subpopulations, there was no detectable association of the pattern with the underlying disease. Despite their distinct developmental stage, all CD21low B cells share a common core phenotype including the increased expression of inhibitory receptors, associated with an elevated constitutive phosphorylation of proximal signaling molecules downstream of the BCR but impaired Ca2+ mobilization and NF-κB activation after BCR stimulation. Further, this was accompanied by impaired upregulation of CD69, although CD86 upregulation was preserved. Beyond maturation-associated differences, the common core characteristics of all CD21low B cell populations suggests either a common ancestry or a shared sustained imprint by the environment they originated in.
2020-09-09T00:00:00Z