Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Ciesek, SandraSteinmann, Eike

Iken, Markus
Ott, Michael
Helfritz, Fabian A
Wappler, Ilka
Manns, Michael P
Wedemeyer, Heiner
Pietschmann, Thomas
Issue Date
2010-05
Metadata
Show full item recordAbstract
BACKGROUND & AIMS: Corticosteroids are used as immunosuppressants in patients with autoimmune disorders and transplant recipients. However, these drugs worsen hepatitis C virus (HCV) recurrence after liver transplantation, suggesting that they may directly exacerbate HCV infection. METHODS: The influence of immunosuppressive drugs on HCV replication, assembly, and entry was assessed in Huh-7.5 cells and primary human hepatocytes using cell culture- and patient-derived HCV. Replication was quantified by immunofluorescence, luciferase assays, quantitative reverse-transcriptase polymerase chain reaction, or core enzyme-linked immunosorbent assays. Expression of HCV entry factors was evaluated by cell sorting and immunoblot analyses. RESULTS: Glucocorticosteroids slightly reduced HCV RNA replication but increased efficiency of HCV entry by up to 10-fold. This was independent of HCV genotype but specific to HCV because vesicular stomatitis virus glycoprotein-dependent infection was not affected by these drugs. The increase in HCV entry was accompanied by up-regulation of messenger RNA and protein levels of occludin and the scavenger receptor class B type I-2 host cell proteins required for HCV infection; increase of entry by glucocorticosteroids was ablated by RU-486, an inhibitor of glucocorticosteroid signaling. Glucocorticosteroids increased propagation of cell culture-derived HCV approximately 5- to 10-fold in partially differentiated human hepatoma cells and increased infection of primary human hepatocytes by cell culture- and patient-derived HCV. CONCLUSIONS: Glucocorticosteroides specifically increase HCV entry by up-regulating the cell entry factors occludin and scavenger receptor class B type I. Our data suggest that the potential effects of high-dose glucocorticosteroids on HCV infection in vivo may be due to increased HCV dissemination.Citation
Glucocorticosteroids increase cell entry by hepatitis C virus. 2010, 138 (5):1875-84 GastroenterologyAffiliation
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany.Journal
GastroenterologyPubMed ID
20152835Type
ArticleLanguage
enISSN
1528-0012ae974a485f413a2113503eed53cd6c53
10.1053/j.gastro.2010.02.004
Scopus Count
The following license files are associated with this item:
Related articles
- A plant-derived flavonoid inhibits entry of all HCV genotypes into human hepatocytes.
- Authors: Haid S, Novodomská A, Gentzsch J, Grethe C, Geuenich S, Bankwitz D, Chhatwal P, Jannack B, Hennebelle T, Bailleul F, Keppler OT, Poenisch M, Bartenschlager R, Hernandez C, Lemasson M, Rosenberg AR, Wong-Staal F, Davioud-Charvet E, Pietschmann T
- Issue date: 2012 Jul
- Entry of pseudotyped hepatitis C virus into primary human hepatocytes depends on the scavenger class B type I receptor.
- Authors: Régeard M, Trotard M, Lepère C, Gripon P, Le Seyec J
- Issue date: 2008 Dec
- Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus.
- Authors: Lavillette D, Tarr AW, Voisset C, Donot P, Bartosch B, Bain C, Patel AH, Dubuisson J, Ball JK, Cosset FL
- Issue date: 2005 Feb
- Interferon alpha decreases expression of human scavenger receptor class BI, a possible HCV receptor in hepatocytes.
- Authors: Murao K, Imachi H, Yu X, Cao WM, Nishiuchi T, Chen K, Li J, Ahmed RA, Wong NC, Ishida T
- Issue date: 2008 May
- Hepatitis C virus enters human peripheral neuroblastoma cells - evidence for extra-hepatic cells sustaining hepatitis C virus penetration.
- Authors: Bürgel B, Friesland M, Koch A, Manns MP, Wedemeyer H, Weissenborn K, Schulz-Schaeffer WJ, Pietschmann T, Steinmann E, Ciesek S
- Issue date: 2011 Aug