Show simple item record

dc.contributor.authorFranzke, Anke
dc.contributor.authorGeffers, Robert
dc.contributor.authorHunger, J Katrin
dc.contributor.authorPförtner, Susanne
dc.contributor.authorPiao, Wenji
dc.contributor.authorIvanyi, Philipp
dc.contributor.authorGrosse, Jens
dc.contributor.authorProbst-Kepper, Michael
dc.contributor.authorGanser, Arnold
dc.contributor.authorBuer, Jan
dc.date.accessioned2007-06-14T12:13:49Z
dc.date.available2007-06-14T12:13:49Z
dc.date.issued2006
dc.identifier.citationBMC Genomics 2006, 7:263en
dc.identifier.issn1471-2164
dc.identifier.pmid17052335
dc.identifier.doi10.1186/1471-2164-7-263
dc.identifier.urihttp://hdl.handle.net/10033/12368
dc.description.abstractBACKGROUND: Aplastic anemia (AA) is a bone marrow failure syndrome mostly characterized by an immune-mediated destruction of marrow hematopoietic progenitor/stem cells. The resulting hypocellularity limits a detailed analysis of the cellular immune response. To overcome this technical problem we performed a microarray analysis of CD3+ T-cells derived from bone marrow aspirates and peripheral blood samples of newly diagnosed AA patients and healthy volunteers. Two AA patients were additionally analyzed after achieving a partial remission following immunosuppression. The regulation of selected candidate genes was confirmed by real-time RT-PCR. RESULTS: Among more than 22,200 transcripts, 583 genes were differentially expressed in the bone marrow of AA patients compared to healthy controls. Dysregulated genes are involved in T-cell mediated cytotoxicity, immune response of Th1 differentiated T-cells, and major regulators of immune function. In hematological remission the expression levels of several candidate genes tend to normalize, such as immune regulators and genes involved in proinflammatory immune response. CONCLUSION: Our study suggests a pivotal role of Th1/Tc1 differentiated T-cells in immune-mediated marrow destruction of AA patients. Most importantly, immune regulatory genes could be identified, which are likely involved in the recovery of hematopoiesis and may help to design new therapeutic strategies in bone marrow failure syndromes.
dc.format.extent-1 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.titleIdentification of novel regulators in T-cell differentiation of aplastic anemia patients.en
dc.typeArticleen
dc.format.digYES
refterms.dateFOA2018-06-12T22:34:51Z
html.description.abstractBACKGROUND: Aplastic anemia (AA) is a bone marrow failure syndrome mostly characterized by an immune-mediated destruction of marrow hematopoietic progenitor/stem cells. The resulting hypocellularity limits a detailed analysis of the cellular immune response. To overcome this technical problem we performed a microarray analysis of CD3+ T-cells derived from bone marrow aspirates and peripheral blood samples of newly diagnosed AA patients and healthy volunteers. Two AA patients were additionally analyzed after achieving a partial remission following immunosuppression. The regulation of selected candidate genes was confirmed by real-time RT-PCR. RESULTS: Among more than 22,200 transcripts, 583 genes were differentially expressed in the bone marrow of AA patients compared to healthy controls. Dysregulated genes are involved in T-cell mediated cytotoxicity, immune response of Th1 differentiated T-cells, and major regulators of immune function. In hematological remission the expression levels of several candidate genes tend to normalize, such as immune regulators and genes involved in proinflammatory immune response. CONCLUSION: Our study suggests a pivotal role of Th1/Tc1 differentiated T-cells in immune-mediated marrow destruction of AA patients. Most importantly, immune regulatory genes could be identified, which are likely involved in the recovery of hematopoiesis and may help to design new therapeutic strategies in bone marrow failure syndromes.


Files in this item

Thumbnail
Name:
Franzke_BMC Genomics_fin.pdf
Size:
-1bytes
Format:
PDF

This item appears in the following Collection(s)

Show simple item record