• Login
    View Item 
    •   Home
    • Division of Molecular Biotechnology (MBIO)
    • Dept. Gene Regulation and Differentiation (RDIF)
    • Publications of Dept. Gene Regulation and Differentiation (RDIF)
    • View Item
    •   Home
    • Division of Molecular Biotechnology (MBIO)
    • Dept. Gene Regulation and Differentiation (RDIF)
    • Publications of Dept. Gene Regulation and Differentiation (RDIF)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of HZICommunitiesTitleAuthorsIssue DateSubmit DateSubjectsJournalTypesSubject (MeSH)This CollectionTitleAuthorsIssue DateSubmit DateSubjectsJournalTypesSubject (MeSH)

    My Account

    LoginRegister

    Local Links

    About: PolicyHelmholtz-Zentrum für Infektionsforschung HomepageHZI-Library HomepageContact usOpen AccessPublishing ApproachGetting StartedEditing ProfileBrowsing OptionsUsing SearchSubmitting ContentLicenced Journals & access details here

    Statistics

    Display statistics

    Six-colour fluorescent imaging of lymphoid tissue based on colour addition theory.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Thumbnail
    Name:
    Templatepage_plus doc_bma.pdf
    Size:
    -1bytes
    Format:
    PDF
    Description:
    main document
    Download
    Thumbnail
    Name:
    Figure1.tif
    Size:
    7.841Mb
    Format:
    TIFF image
    Description:
    figure1
    Download
    Thumbnail
    Name:
    Figure2.tif
    Size:
    7.147Mb
    Format:
    TIFF image
    Description:
    figure2
    Download
    Thumbnail
    Name:
    Figure3.tif
    Size:
    -1bytes
    Format:
    TIFF image
    Description:
    figure3
    Download
    Thumbnail
    Name:
    Figure4.tif
    Size:
    11.93Mb
    Format:
    TIFF image
    Description:
    figure4
    Download
    Thumbnail
    Name:
    Figure5.tif
    Size:
    -1bytes
    Format:
    TIFF image
    Description:
    figure5
    Download
    Thumbnail
    Name:
    Figure_6.eps
    Size:
    -1bytes
    Format:
    Postscript
    Description:
    figure6
    Download
    Thumbnail
    Name:
    S Figure 1a.tif
    Size:
    -1bytes
    Format:
    TIFF image
    Description:
    Sfigure1a
    Download
    Thumbnail
    Name:
    S Figure 1b.tif
    Size:
    125.0Kb
    Format:
    TIFF image
    Description:
    Sfigure1b
    Download
    Thumbnail
    Name:
    S Figure 2a.tif
    Size:
    -1bytes
    Format:
    TIFF image
    Description:
    Sfigure2a
    Download
    Thumbnail
    Name:
    S Figure 2b.tif
    Size:
    -1bytes
    Format:
    TIFF image
    Description:
    Sfigure2b
    Download
    Thumbnail
    Name:
    Supplementary Table 1.doc
    Size:
    -1bytes
    Format:
    Microsoft Word
    Description:
    supplementary Table1
    DownloadPDF Variant
    Thumbnail
    Name:
    Supplementary Table 2.doc
    Size:
    -1bytes
    Format:
    Microsoft Word
    Description:
    Supplementary Table2
    DownloadPDF Variant
    Thumbnail
    Name:
    Table 1.doc
    Size:
    -1bytes
    Format:
    Microsoft Word
    Description:
    Table1
    DownloadPDF Variant
    Thumbnail
    Name:
    Table 2.doc
    Size:
    -1bytes
    Format:
    Microsoft Word
    Description:
    Table2
    DownloadPDF Variant
    Thumbnail
    Name:
    S video1.avi
    Size:
    338Kb
    Format:
    Unknown
    Description:
    Svideo1
    Download
    Thumbnail
    Name:
    S video 2.avi
    Size:
    708Kb
    Format:
    Unknown
    Description:
    Svideo2
    Download
    View more filesView fewer files
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Ma, Bin
    Winkelbach, Simon
    Lindenmaier, Werner
    Dittmar, Kurt E J
    Issue Date
    2006
    
    Metadata
    Show full item record
    Abstract
    Multi-colour imaging of immunofluorescently labelled tissue using confocal microscopy was accomplished by using colour addition theory. This new technique includes several improvements for immunolabelling: (1) the co-localization of two or more markers on one cell for the identification of specific cell populations; (2) the co-localization of two fluorescent dyes from secondary reagents for the identification of the cells; (3) a multi-step staining protocol with two primary antibodies originating from the same host species or with two or three biotin-conjugated primary antibodies. After image acquisition, colour segmentation/unmixing are applied to the single multi-colour image to generate multi-pseudo-channels for individual or co-localized fluorescent dyes. With this new technique, we have been able to visualize six cell populations simultaneously in the mouse lymph node and intestine. The efficiency of this method has also been demonstrated in the three-dimensional reconstruction of thick sections from mouse ileum. Our method is simple, efficient, and may be indispensable in experimental cell and tissue studies requiring multiple immunolabelling.
    Citation
    Acta Histochem. 2006, 108(4):243-57
    URI
    http://hdl.handle.net/10033/12399
    DOI
    10.1016/j.acthis.2006.02.002
    PubMed ID
    16730369
    Type
    Article
    Language
    en
    ISSN
    0065-1281
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.acthis.2006.02.002
    Scopus Count
    Collections
    Publications of Dept. Gene Regulation and Differentiation (RDIF)

    entitlement

    Related articles

    • Six-color segmentation of multicolor images in the infection studies of Listeria monocytogenes.
    • Authors: Ma B, He F, Jablonska J, Winkelbach S, Lindenmaier W, Zeng AP, Dittmar KE
    • Issue date: 2007 Feb
    • Automatic registration of serial sections of mouse lymph node by using Image-Reg.
    • Authors: Ma B, Lin Z, Winkelbach S, Lindenmaier W, Dittmar KE
    • Issue date: 2008 Jun
    • Direct immunofluorescent labeling of cells.
    • Authors: Pástor MV
    • Issue date: 2010
    • Tyramide signal amplification method in multiple-label immunofluorescence confocal microscopy.
    • Authors: Wang G, Achim CL, Hamilton RL, Wiley CA, Soontornniyomkij V
    • Issue date: 1999 Aug
    • Experimental induction and three-dimensional two-photon imaging of conjunctiva-associated lymphoid tissue.
    • Authors: Steven P, Rupp J, Hüttmann G, Koop N, Lensing C, Laqua H, Gebert A
    • Issue date: 2008 Apr

    DSpace software copyright © 2002-2025  DuraSpace
    Quick Guide | Kontakt | Feedback abschicken
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.