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dc.contributor.authorWang, X
dc.contributor.authorRochon, M
dc.contributor.authorLamprokostopoulou, A
dc.contributor.authorLünsdorf, H
dc.contributor.authorNimtz, M
dc.contributor.authorRömling, U
dc.date.accessioned2007-07-02T08:59:50Z
dc.date.available2007-07-02T08:59:50Z
dc.date.issued2006-10-01
dc.identifier.citationCell. Mol. Life Sci. 2006, 63(19-20):2352-63en
dc.identifier.issn1420-682X
dc.identifier.pmid16952050
dc.identifier.doi10.1007/s00018-006-6222-4
dc.identifier.urihttp://hdl.handle.net/10033/12489
dc.description.abstractCommensal Escherichia coli form biofilms at body temperature by expressing the extracellular matrix components curli fimbriae and cellulose. The role of curli fimbriae and cellulose in the interaction of commensal E. coli with the intestinal epithelial cell line HT-29 was investigated. Expression of curli fimbriae by the typical commensal isolate E. coli TOB1 caused adherence and internalization of the bacteria and triggered IL-8 production in HT-29 cells. In particular, induction of IL-8 production was complex and involved curli-bound flagellin. While cellulose alone had no effect on the interaction of TOB1 with HT-29 cells, co-expression of cellulose with curli fimbriae decreased adherence to, internalization and IL-8 induction of HT-29 cells. Investigation of a panel of commensal isolates showed a partial correlation between expression of curli fimbriae and enhanced internalization and IL-8 production. In addition, a high immunostimulatory flagellin was identified. Thus, the consequences of expression of extracellular matrix components on commensal bacterial-host interactions are complex.
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dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.titleImpact of biofilm matrix components on interaction of commensal Escherichia coli with the gastrointestinal cell line HT-29.en
dc.typeArticleen
dc.format.digYES
refterms.dateFOA2018-06-13T00:28:59Z
html.description.abstractCommensal Escherichia coli form biofilms at body temperature by expressing the extracellular matrix components curli fimbriae and cellulose. The role of curli fimbriae and cellulose in the interaction of commensal E. coli with the intestinal epithelial cell line HT-29 was investigated. Expression of curli fimbriae by the typical commensal isolate E. coli TOB1 caused adherence and internalization of the bacteria and triggered IL-8 production in HT-29 cells. In particular, induction of IL-8 production was complex and involved curli-bound flagellin. While cellulose alone had no effect on the interaction of TOB1 with HT-29 cells, co-expression of cellulose with curli fimbriae decreased adherence to, internalization and IL-8 induction of HT-29 cells. Investigation of a panel of commensal isolates showed a partial correlation between expression of curli fimbriae and enhanced internalization and IL-8 production. In addition, a high immunostimulatory flagellin was identified. Thus, the consequences of expression of extracellular matrix components on commensal bacterial-host interactions are complex.


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