Susceptibility to experimental biliary atresia linked to different hepatic gene expression profiles in two mouse strains.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Kuebler, Joachim F
MetadataShow full item record
AbstractAim: To compare hepatic gene expression during the development of experimental biliary atresia (BA) in two different mouse strains. Methods: Balb/c mice and C57Black/6 (Black/6) mice were infected with rhesus rotavirus (RRV) postpartum, clinical signs of BA and survival were noted. Liver sections were assessed for cluster of differentiation antigen (CD) 3, CD4 and CD8 expression, and the hepatic virus load was determined. Second, mice of both strains were sacrificed three days after infection. Isolated hepatic RNA was subjected to gene expression analysis using Affymetrix Gene Chip MOE 430 2.0. Results: The incidence of BA was significantly lower in Black/6 mice compared to Balb/c mice (13.5% vs. 67%, P < 0.05). The mean virus titers were higher in mice with BA compared to mice without BA. Different gene profiles three days after virus infection were noted, with differential expression of 201 genes, including those regulating apoptosis, nucleic acid binding, transport function and particularly the immune response (chemokine C-C motif ligand 2, toll-like receptor 3, CD antigen 14, chemokine (C-X-C motif) ligands 10 and 11). This correlated with a significant increase of CD4 positive cells only in Balb/c mice with BA compared to healthy mice (13.5 vs. 5.0; P < 0.05). Black/6 mice did not exhibit any significant increase of CD3 or CD4 leukocytes despite cholestasis. Conclusion: The different susceptibility to experimental BA was associated with an increase of CD4 T-cells in the liver of Balb/c mice, which is linked to different gene profiles at the onset of bile duct obstruction.
CitationSusceptibility to experimental biliary atresia linked to different hepatic gene expression profiles in two mouse strains. 2010, 40 (2):196-203 Hepatol. Res.
AffiliationDepartment of Pediatric Surgery, Hannover Medical School, Hannover, Germany.
The following license files are associated with this item:
- Functional Mx protein does not prevent experimental biliary atresia in Balb/c mice.
- Authors: Wehrmann F, Kuebler JF, Wienecke S, Al-Masri AN, Petersen C, Leonhardt J
- Issue date: 2008 Oct
- Preconceptional oral vaccination prevents experimental biliary atresia in newborn mice.
- Authors: Turowski C, Leonhardt J, Teichmann B, Heim A, Baumann U, Kuebler JF, Petersen C
- Issue date: 2010 May
- CD8+ T lymphocyte response against extrahepatic biliary epithelium is activated by epitopes within NSP4 in experimental biliary atresia.
- Authors: Zheng S, Zhang H, Zhang X, Peng F, Chen X, Yang J, Brigstock D, Feng J
- Issue date: 2014 Jul 15
- Rotavirus particles in the extrahepatic bile duct in experimental biliary atresia.
- Authors: Oetzmann von Sochaczewski C, Pintelon I, Brouns I, Dreier A, Klemann C, Timmermans JP, Petersen C, Kuebler JF
- Issue date: 2014 Apr
- B cell deficient mice are protected from biliary obstruction in the rotavirus-induced mouse model of biliary atresia.
- Authors: Feldman AG, Tucker RM, Fenner EK, Pelanda R, Mack CL
- Issue date: 2013