Potentiation of epithelial innate host responses by intercellular communication.
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Authors
Dolowschiak, TamasChassin, Cécilia
Ben Mkaddem, Sanae
Fuchs, Thilo M
Weiss, Siegfried
Vandewalle, Alain
Hornef, Mathias W
Issue Date
2010
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Show full item recordAbstract
The epithelium efficiently attracts immune cells upon infection despite the low number of pathogenic microbes and moderate levels of secreted chemokines per cell. Here we examined whether horizontal intercellular communication between cells may contribute to a coordinated response of the epithelium. Listeria monocytogenes infection, transfection, and microinjection of individual cells within a polarized intestinal epithelial cell layer were performed and activation was determined at the single cell level by fluorescence microscopy and flow cytometry. Surprisingly, chemokine production after L. monocytogenes infection was primarily observed in non-infected epithelial cells despite invasion-dependent cell activation. Whereas horizontal communication was independent of gap junction formation, cytokine secretion, ion fluxes, or nitric oxide synthesis, NADPH oxidase (Nox) 4-dependent oxygen radical formation was required and sufficient to induce indirect epithelial cell activation. This is the first report to describe epithelial cell-cell communication in response to innate immune activation. Epithelial communication facilitates a coordinated infectious host defence at the very early stage of microbial infection.Citation
Potentiation of epithelial innate host responses by intercellular communication. 2010, 6 (11):e1001194 PLoS Pathog.Affiliation
Hannover Medical School, Hannover, Germany.Journal
PLoS pathogensPubMed ID
21124989Type
ArticleLanguage
enISSN
1553-7374ae974a485f413a2113503eed53cd6c53
10.1371/journal.ppat.1001194
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