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dc.contributor.authorGross, Frank
dc.contributor.authorLuniak, Nora
dc.contributor.authorPerlova, Olena
dc.contributor.authorGaitatzis, Nikolaos
dc.contributor.authorJenke-Kodama, Holger
dc.contributor.authorGerth, Klaus
dc.contributor.authorGottschalk, Daniela
dc.contributor.authorDittmann, Elke
dc.contributor.authorMüller, Rolf
dc.date.accessioned2007-09-25T11:27:40Z
dc.date.available2007-09-25T11:27:40Z
dc.date.issued2006-03-01
dc.identifier.citationArch. Microbiol. 2006, 185(1):28-38en
dc.identifier.issn0302-8933
dc.identifier.pmid16395556
dc.identifier.doi10.1007/s00203-005-0059-3
dc.identifier.urihttp://hdl.handle.net/10033/13787
dc.description.abstractType III polyketide synthases (PKS) were regarded as typical for plant secondary metabolism before they were found in microorganisms recently. Due to microbial genome sequencing efforts, more and more type III PKS are found, most of which of unknown function. In this manuscript, we report a comprehensive analysis of the phylogeny of bacterial type III PKS and report the expression of a type III PKS from the myxobacterium Sorangium cellulosum in pseudomonads. There is no precedent of a secondary metabolite that might be biosynthetically correlated to a type III PKS from any myxobacterium. Additionally, an inactivation mutant of the S. cellulosum gene shows no physiological difference compared to the wild-type strain which is why these type III PKS are assumed to be "silent" under the laboratory conditions administered. One type III PKS (SoceCHS1) was expressed in different Pseudomonas sp. after the heterologous expression in Escherichia coli failed. Cultures of recombinant Pseudomonas sp. harbouring SoceCHS1 turned red upon incubation and the diffusible pigment formed was identified as 2,5,7-trihydroxy-1,4-naphthoquinone, the autooxidation product of 1,3,6,8-tetrahydroxynaphthalene. The successful heterologous production of a secondary metabolite using a gene not expressed under administered laboratory conditions provides evidence for the usefulness of our approach to activate such secondary metabolite genes for the production of novel metabolites.
dc.format.extent616352 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.titleBacterial type III polyketide synthases: phylogenetic analysis and potential for the production of novel secondary metabolites by heterologous expression in pseudomonads.en
dc.typeArticleen
dc.format.digYES
refterms.dateFOA2018-06-12T17:24:39Z
html.description.abstractType III polyketide synthases (PKS) were regarded as typical for plant secondary metabolism before they were found in microorganisms recently. Due to microbial genome sequencing efforts, more and more type III PKS are found, most of which of unknown function. In this manuscript, we report a comprehensive analysis of the phylogeny of bacterial type III PKS and report the expression of a type III PKS from the myxobacterium Sorangium cellulosum in pseudomonads. There is no precedent of a secondary metabolite that might be biosynthetically correlated to a type III PKS from any myxobacterium. Additionally, an inactivation mutant of the S. cellulosum gene shows no physiological difference compared to the wild-type strain which is why these type III PKS are assumed to be "silent" under the laboratory conditions administered. One type III PKS (SoceCHS1) was expressed in different Pseudomonas sp. after the heterologous expression in Escherichia coli failed. Cultures of recombinant Pseudomonas sp. harbouring SoceCHS1 turned red upon incubation and the diffusible pigment formed was identified as 2,5,7-trihydroxy-1,4-naphthoquinone, the autooxidation product of 1,3,6,8-tetrahydroxynaphthalene. The successful heterologous production of a secondary metabolite using a gene not expressed under administered laboratory conditions provides evidence for the usefulness of our approach to activate such secondary metabolite genes for the production of novel metabolites.


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