Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production.
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
2006
Metadata
Show full item recordAbstract
BACKGROUND: Retroviral Gag determines virus assembly at the plasma membrane and the formation of virus-like particles in intracellular multivesicular bodies. Thereby, retroviruses exploit by interaction with cellular partners the cellular machineries for vesicular transport in various ways. RESULTS: The retroviral Gag precursor protein drives assembly of murine leukaemia viruses (MLV) at the plasma membrane (PM) and the formation of virus like particles in multivesicular bodies (MVBs). In our study we show that caveolin-1 (Cav-1), a multifunctional membrane-associated protein, co-localizes with Gag in a punctate pattern at the PM of infected NIH 3T3 cells. We provide evidence that Cav-1 interacts with the matrix protein (MA) of the Gag precursor. This interaction is mediated by a Cav-1 binding domain (CBD) within the N-terminus of MA. Interestingly, the CBD motif identified within MA is highly conserved among most other gamma-retroviruses. Furthermore, Cav-1 is incorporated into MLV released from NIH 3T3 cells. Overexpression of a GFP fusion protein containing the putative CBD of the retroviral MA resulted in a considerable decrease in production of infectious retrovirus. Moreover, expression of a dominant-negative Cav-1 mutant affected retroviral titres significantly. CONCLUSION: This study demonstrates that Cav-1 interacts with MLV Gag, co-localizes with Gag at the PM and affects the production of infectious virus. The results strongly suggest a role for Cav-1 in the process of virus assembly.Citation
Virol. J. 2006, 3:73PubMed ID
16956408Type
ArticleLanguage
enISSN
1743-422Xae974a485f413a2113503eed53cd6c53
10.1186/1743-422X-3-73
Scopus Count
The following license files are associated with this item:
Related articles
- Inhibition of viral assembly in murine cells by HIV-1 matrix.
- Authors: Hübner W, Chen BK
- Issue date: 2006 Aug 15
- Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses.
- Authors: Sandrin V, Muriaux D, Darlix JL, Cosset FL
- Issue date: 2004 Jul
- Caveola-dependent endocytic entry of amphotropic murine leukemia virus.
- Authors: Beer C, Andersen DS, Rojek A, Pedersen L
- Issue date: 2005 Aug
- Intracellular assembly and budding of the Murine Leukemia Virus in infected cells.
- Authors: Houzet L, Gay B, Morichaud Z, Briant L, Mougel M
- Issue date: 2006 Feb 10
- Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains.
- Authors: Beer C, Pedersen L, Wirth M
- Issue date: 2005 Apr 19