• Login
    View Item 
    •   Home
    • Division of Cell and Immune Biology (ZIB)
    • RG Mucosal Immunity (MI)
    • Publications of RG Mucosal Immunity (MI)
    • View Item
    •   Home
    • Division of Cell and Immune Biology (ZIB)
    • RG Mucosal Immunity (MI)
    • Publications of RG Mucosal Immunity (MI)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of HZICommunitiesTitleAuthorsIssue DateSubmit DateSubjectsJournalTypesSubject (MeSH)This CollectionTitleAuthorsIssue DateSubmit DateSubjectsJournalTypesSubject (MeSH)

    My Account

    LoginRegister

    Local Links

    About: PolicyHelmholtz-Zentrum für Infektionsforschung HomepageHZI-Library HomepageContact usOpen AccessPublishing ApproachGetting StartedEditing ProfileBrowsing OptionsUsing SearchSubmitting Content

    Statistics

    Display statistics

    Multiple synergizing factors contribute to the strength of the CD8+ T cell response against listeriolysin O.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Bruder et al_final.pdf
    Size:
    350.5Kb
    Format:
    PDF
    Description:
    original document
    Download
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Bruder, Dunja cc
    Nussbaum, Alexander K
    Gakamsky, Dimitry M
    Schirle, Markus
    Stevanovic, Stefan
    Singh-Jasuja, Harpreet
    Darji, Ayub
    Chakraborty, Trinad
    Schild, Hansjörg
    Pecht, Israel
    Weiss, Siegfried
    Show allShow less
    Issue Date
    2006-01-01
    
    Metadata
    Show full item record
    Abstract
    Immunodominance in CD8+ T cell responses against Listeria monocytogenes is a well-recognized but still not fully understood phenomenon. From listeriolysin, the major virulence factor of L. monocytogenes, only a single epitope, pLLO91-99, is presented by MHC class I molecules in BALB/c mice which dominates the cytotoxic T cell response against this bacterial pathogen. To obtain more insights into the molecular and cellular mechanisms underlying immunodominance of this particular epitope, we compared the various steps involved in the presentation and recognition of pLLO91-99 derived from a wild-type toxin with an equivalent epitope from a mutated toxin. This fully functional variant contains within the pLLO91-99 epitope a conservative isoleucine to alanine replacement at the C-terminal anchor residue which results in loss of antigenicity. The binding properties of the variant peptide to soluble Kd remained unaffected and cytotoxic T cells capable of recognizing the pLLO99A/Kd complex were detectable in BALB/c mice. However, such T cells required higher concentrations of antigen in order to be optimally activated in vitro. A comparison between the TAP translocation efficiency of wild-type and mutant peptide demonstrated that the mutation at the C-terminus leads to a reduced transportation rate. Furthermore, the amino acid substitution changes the in vitro proteasomal cleavage pattern, resulting in a reduced liberation of the correct peptide from a polypeptide precursor. Thus, in all assays employed the immunodominant epitope performs optimally while the variant was found to be inferior. The synergy of all these steps most likely is the decisive factor in the immunodominance of pLLO91-99.
    Citation
    Int. Immunol. 2006, 18(1):89-100
    URI
    http://hdl.handle.net/10033/14546
    DOI
    10.1093/intimm/dxh352
    PubMed ID
    16291651
    Type
    Article
    Language
    en
    ISSN
    0953-8178
    ae974a485f413a2113503eed53cd6c53
    10.1093/intimm/dxh352
    Scopus Count
    Collections
    Publications of RG Mucosal Immunity (MI)

    entitlement

    Related articles

    • Elimination of the listeriolysin O-directed immune response by conservative alteration of the immunodominant listeriolysin O amino acid 91 to 99 epitope.
    • Authors: Bouwer HG, Moors M, Hinrichs DJ
    • Issue date: 1996 Sep
    • Cytotoxic-T-lymphocyte responses to epitopes of listeriolysin O and p60 following infection with Listeria monocytogenes.
    • Authors: Bouwer HG, Hinrichs DJ
    • Issue date: 1996 Jul
    • Adenovirus-based vaccine against Listeria monocytogenes: extending the concept of invariant chain linkage.
    • Authors: Jensen S, Steffensen MA, Jensen BA, Schlüter D, Christensen JP, Thomsen AR
    • Issue date: 2013 Oct 15
    • Exploring the role of the CTL epitope region of listeriolysin O in the pathogenesis of Listeria monocytogenes.
    • Authors: Lety MA, Frehel C, Raynaud C, Dupuis M, Charbit A
    • Issue date: 2006 May
    • A novel approach of direct ex vivo epitope mapping identifies dominant and subdominant CD4 and CD8 T cell epitopes from Listeria monocytogenes.
    • Authors: Geginat G, Schenk S, Skoberne M, Goebel W, Hof H
    • Issue date: 2001 Feb 1

    DSpace software copyright © 2002-2019  DuraSpace
    Quick Guide | Kontakt | Feedback abschicken
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.