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    Identification of new acceptor specificities of glycosyltransferase R with the aid of substrate microarrays.

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    Authors
    Seibel, Jürgen
    Hellmuth, Hendrik
    Hofer, Bernd
    Kicinska, Anna-Maria
    Schmalbruch, Bodo
    Issue Date
    2006-02-01
    
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    Abstract
    Finding opportunities to construct sugar motifs and to transfer them to targets of biological relevance and rapid identification of glycosylation events are important goals for glycobiology and a field of increasing interest. Here we have applied an enzyme microarray screening system for the identification of new acceptor specificities of the glycosyltransferase R (GTFR) from Streptococcus oralis (E.C. 2.4.1.5), which was able to effect the synthesis of sugar motifs in short times and with low amounts of substrate. These observations resulted in the development of a convenient alpha-glycosylation by the non-Leloir glycosyltransferase GTFR, with sucrose as substrate and with different alcohols and amino acid derivatives as acceptors, for the synthesis of glycoethers and glycosylated amino acids not observed with the use of familiar GTFs with high sequence homology.
    Citation
    Chembiochem 2006, 7(2):310-20
    URI
    http://hdl.handle.net/10033/14630
    DOI
    10.1002/cbic.200500350
    PubMed ID
    16416490
    Type
    Article
    Language
    en
    ISSN
    1439-4227
    ae974a485f413a2113503eed53cd6c53
    10.1002/cbic.200500350
    Scopus Count
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