• Login
    View Item 
    •   Home
    • Division of Molekulare Strukurbiologie (MOSB)
    • Publications from Division of Molekulare Struktur Biologie (MOSB)
    • View Item
    •   Home
    • Division of Molekulare Strukurbiologie (MOSB)
    • Publications from Division of Molekulare Struktur Biologie (MOSB)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of HZICommunitiesTitleAuthorsIssue DateSubmit DateSubjectsJournalTypesSubject (MeSH)This CollectionTitleAuthorsIssue DateSubmit DateSubjectsJournalTypesSubject (MeSH)

    My Account

    LoginRegister

    Local Links

    About: PolicyHelmholtz-Zentrum für Infektionsforschung HomepageHZI-Library HomepageContact usOpen AccessPublishing ApproachGetting StartedEditing ProfileBrowsing OptionsUsing SearchSubmitting ContentLicenced Journals & access details here

    Statistics

    Display statistics

    Synthesis of sucrose analogues and the mechanism of action of Bacillus subtilis fructosyltransferase (levansucrase).

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Thumbnail
    Name:
    Seibel_Hecht_final.pdf
    Size:
    1011.Kb
    Format:
    PDF
    Description:
    original document
    Download
    Average rating
     
       votes
    Cast your vote
    You can rate an item by clicking the amount of stars they wish to award to this item. When enough users have cast their vote on this item, the average rating will also be shown.
    Star rating
     
    Your vote was cast
    Thank you for your feedback
    Authors
    Seibel, Jürgen
    Moraru, Roxana
    Götze, Sven
    Buchholz, Klaus
    Na'amnieh, Shukrallah
    Pawlowski, Alice
    Hecht, Hans-Jürgen
    Issue Date
    2006-10-16
    
    Metadata
    Show full item record
    Abstract
    In the present study, we have coupled detailed acceptor and donor substrate studies of the fructosyltransferase (FTF, levansucrase) (EC 2.4.1.162) from Bacillus subtilis NCIMB 11871, with a structural model of the substrate enzyme complex in order to investigate in detail the roles of the active site amino acids in the catalytic action of the enzyme and the scope and limitation of substrates. Therefore we have isolated the ftf gene, expressed in Escherichia coli, yielding a levansucrase. Consequently, detailed acceptor property effects in the fructosylation by systematic variation of glycoside acceptors with respect to the positions (2, 3, 4 and 6) of the hydroxyl groups from equatorial to axial have been studied for preparative scale production of new oligosaccharides. Such investigations provided mechanistic insights of the FTF reaction. The configuration and the presence of the C-2 and C-3 hydroxyl groups of the glucopyranoside derivatives either as substrates or acceptors have been identified to be rate limiting for the trans-fructosylation process. The rates are rationalized on the basis of the coordination of d-glycopyranoside residues in (4)C(1) conformation with a network of amino acids by Arg360, Tyr411, Glu342, Trp85, Asp247 and Arg246 stabilization of both acceptors and substrates. In addition we also describe the first FTF reaction, which catalyzes the beta-(1-->2)-fructosyl transfer to 2-OH of L-sugars (L-glucose, L-rhamnose, L-galactose, L-fucose, L-xylose) presumably in a (1)C(4) conformation. In those conformations, the L-glycopyranosides are stabilized by the same hydrogen network. Structures of the acceptor products were determined by NMR and mass spectrometry analysis.
    Citation
    Carbohydr. Res. 2006, 341(14):2335-49
    URI
    http://hdl.handle.net/10033/14631
    DOI
    10.1016/j.carres.2006.07.001
    PubMed ID
    16870166
    Type
    Article
    Language
    en
    ISSN
    0008-6215
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.carres.2006.07.001
    Scopus Count
    Collections
    Publications from Division of Molekulare Struktur Biologie (MOSB)

    entitlement

    Related articles

    • Investigations of the transfructosylation reaction by fructosyltransferase from B. subtilis NCIMB 11871 for the synthesis of the sucrose analogue galactosyl-fructoside.
    • Authors: Baciu IE, Jördening HJ, Seibel J, Buchholz K
    • Issue date: 2005 Apr 6
    • Structural framework of fructosyl transfer in Bacillus subtilis levansucrase.
    • Authors: Meng G, Fütterer K
    • Issue date: 2003 Nov
    • Donor substrate recognition in the raffinose-bound E342A mutant of fructosyltransferase Bacillus subtilis levansucrase.
    • Authors: Meng G, Fütterer K
    • Issue date: 2008 Mar 17
    • Dissecting the catalytic mechanism of a plant beta-D-glucan glucohydrolase through structural biology using inhibitors and substrate analogues.
    • Authors: Hrmova M, Fincher GB
    • Issue date: 2007 Sep 3
    • Lactosucrose production by various microorganisms harboring levansucrase activity.
    • Authors: Park NH, Choi HJ, Oh DK
    • Issue date: 2005 Apr

    DSpace software copyright © 2002-2022  DuraSpace
    Quick Guide | Kontakt | Feedback abschicken
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.