Show simple item record

dc.contributor.authorSeibel, Jürgen
dc.contributor.authorMoraru, Roxana
dc.contributor.authorGötze, Sven
dc.contributor.authorBuchholz, Klaus
dc.contributor.authorNa'amnieh, Shukrallah
dc.contributor.authorPawlowski, Alice
dc.contributor.authorHecht, Hans-Jürgen
dc.date.accessioned2007-11-19T15:29:22Z
dc.date.available2007-11-19T15:29:22Z
dc.date.issued2006-10-16
dc.identifier.citationCarbohydr. Res. 2006, 341(14):2335-49en
dc.identifier.issn0008-6215
dc.identifier.pmid16870166
dc.identifier.doi10.1016/j.carres.2006.07.001
dc.identifier.urihttp://hdl.handle.net/10033/14631
dc.description.abstractIn the present study, we have coupled detailed acceptor and donor substrate studies of the fructosyltransferase (FTF, levansucrase) (EC 2.4.1.162) from Bacillus subtilis NCIMB 11871, with a structural model of the substrate enzyme complex in order to investigate in detail the roles of the active site amino acids in the catalytic action of the enzyme and the scope and limitation of substrates. Therefore we have isolated the ftf gene, expressed in Escherichia coli, yielding a levansucrase. Consequently, detailed acceptor property effects in the fructosylation by systematic variation of glycoside acceptors with respect to the positions (2, 3, 4 and 6) of the hydroxyl groups from equatorial to axial have been studied for preparative scale production of new oligosaccharides. Such investigations provided mechanistic insights of the FTF reaction. The configuration and the presence of the C-2 and C-3 hydroxyl groups of the glucopyranoside derivatives either as substrates or acceptors have been identified to be rate limiting for the trans-fructosylation process. The rates are rationalized on the basis of the coordination of d-glycopyranoside residues in (4)C(1) conformation with a network of amino acids by Arg360, Tyr411, Glu342, Trp85, Asp247 and Arg246 stabilization of both acceptors and substrates. In addition we also describe the first FTF reaction, which catalyzes the beta-(1-->2)-fructosyl transfer to 2-OH of L-sugars (L-glucose, L-rhamnose, L-galactose, L-fucose, L-xylose) presumably in a (1)C(4) conformation. In those conformations, the L-glycopyranosides are stabilized by the same hydrogen network. Structures of the acceptor products were determined by NMR and mass spectrometry analysis.
dc.format.extent1035891 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.titleSynthesis of sucrose analogues and the mechanism of action of Bacillus subtilis fructosyltransferase (levansucrase).en
dc.typeArticleen
dc.format.digYES
refterms.dateFOA2018-06-13T01:05:48Z
html.description.abstractIn the present study, we have coupled detailed acceptor and donor substrate studies of the fructosyltransferase (FTF, levansucrase) (EC 2.4.1.162) from Bacillus subtilis NCIMB 11871, with a structural model of the substrate enzyme complex in order to investigate in detail the roles of the active site amino acids in the catalytic action of the enzyme and the scope and limitation of substrates. Therefore we have isolated the ftf gene, expressed in Escherichia coli, yielding a levansucrase. Consequently, detailed acceptor property effects in the fructosylation by systematic variation of glycoside acceptors with respect to the positions (2, 3, 4 and 6) of the hydroxyl groups from equatorial to axial have been studied for preparative scale production of new oligosaccharides. Such investigations provided mechanistic insights of the FTF reaction. The configuration and the presence of the C-2 and C-3 hydroxyl groups of the glucopyranoside derivatives either as substrates or acceptors have been identified to be rate limiting for the trans-fructosylation process. The rates are rationalized on the basis of the coordination of d-glycopyranoside residues in (4)C(1) conformation with a network of amino acids by Arg360, Tyr411, Glu342, Trp85, Asp247 and Arg246 stabilization of both acceptors and substrates. In addition we also describe the first FTF reaction, which catalyzes the beta-(1-->2)-fructosyl transfer to 2-OH of L-sugars (L-glucose, L-rhamnose, L-galactose, L-fucose, L-xylose) presumably in a (1)C(4) conformation. In those conformations, the L-glycopyranosides are stabilized by the same hydrogen network. Structures of the acceptor products were determined by NMR and mass spectrometry analysis.


Files in this item

Thumbnail
Name:
Publisher version
Thumbnail
Name:
Seibel_Hecht_final.pdf
Size:
1011.Kb
Format:
PDF
Description:
original document

This item appears in the following Collection(s)

Show simple item record