Mutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2.
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Authors
Sabirova, Julia SFerrer, Manuel
Lünsdorf, Heinrich
Wray, Victor
Kalscheuer, Rainer
Steinbüchel, Alexander
Timmis, Kenneth N
Golyshin, Peter N
Issue Date
2006-12
Metadata
Show full item recordAbstract
A novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important polymer when grown on alkanes. The tesB-like gene encodes a distinct novel enzyme activity, which acts exclusively on hydroxylated acyl-CoAs and thus represents a hydroxyacyl-CoA-specific thioesterase. Inactivation of this enzyme results in the rechanneling of CoA-activated hydroxylated fatty acids, the cellular intermediates of alkane degradation, towards PHA production. These findings may open up new avenues for the development of simplified biotechnological processes for the production of PHA as a raw material for the production of bioplastics.Citation
Mutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2. 2006, 188 (24):8452-9 J. Bacteriol.Affiliation
Department of Environmental Microbiology, HZI-Helmholtz Center fro Infection Research, Braunschweig, Germany. jsa05@helmholtz-hzi.deJournal
Journal of bacteriologyPubMed ID
16997960Type
ArticleLanguage
enISSN
0021-9193ae974a485f413a2113503eed53cd6c53
10.1128/JB.01321-06
Scopus Count