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dc.contributor.authorSabirova, Julia S
dc.contributor.authorFerrer, Manuel
dc.contributor.authorLünsdorf, Heinrich
dc.contributor.authorWray, Victor
dc.contributor.authorKalscheuer, Rainer
dc.contributor.authorSteinbüchel, Alexander
dc.contributor.authorTimmis, Kenneth N
dc.contributor.authorGolyshin, Peter N
dc.date.accessioned2007-12-11T09:08:51Z
dc.date.available2007-12-11T09:08:51Z
dc.date.issued2006-12
dc.identifier.citationMutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2. 2006, 188 (24):8452-9 J. Bacteriol.en
dc.identifier.issn0021-9193
dc.identifier.pmid16997960
dc.identifier.doi10.1128/JB.01321-06
dc.identifier.urihttp://hdl.handle.net/10033/15112
dc.description.abstractA novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important polymer when grown on alkanes. The tesB-like gene encodes a distinct novel enzyme activity, which acts exclusively on hydroxylated acyl-CoAs and thus represents a hydroxyacyl-CoA-specific thioesterase. Inactivation of this enzyme results in the rechanneling of CoA-activated hydroxylated fatty acids, the cellular intermediates of alkane degradation, towards PHA production. These findings may open up new avenues for the development of simplified biotechnological processes for the production of PHA as a raw material for the production of bioplastics.
dc.language.isoenen
dc.subject.meshAcyl Coenzyme Aen
dc.subject.meshAlkanesen
dc.subject.meshBiotechnologyen
dc.subject.meshCulture Mediaen
dc.subject.meshDNA Transposable Elementsen
dc.subject.meshEscherichia colien
dc.subject.meshGammaproteobacteriaen
dc.subject.meshMicroscopy, Electron, Scanningen
dc.subject.meshMutationen
dc.subject.meshPolyestersen
dc.subject.meshSubstrate Specificityen
dc.subject.meshThiolester Hydrolasesen
dc.subject.meshUp-Regulationen
dc.titleMutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2.en
dc.typeArticleen
dc.contributor.departmentDepartment of Environmental Microbiology, HZI-Helmholtz Center fro Infection Research, Braunschweig, Germany. jsa05@helmholtz-hzi.de
dc.identifier.journalJournal of bacteriologyen
refterms.dateFOA2018-06-13T09:05:00Z
html.description.abstractA novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important polymer when grown on alkanes. The tesB-like gene encodes a distinct novel enzyme activity, which acts exclusively on hydroxylated acyl-CoAs and thus represents a hydroxyacyl-CoA-specific thioesterase. Inactivation of this enzyme results in the rechanneling of CoA-activated hydroxylated fatty acids, the cellular intermediates of alkane degradation, towards PHA production. These findings may open up new avenues for the development of simplified biotechnological processes for the production of PHA as a raw material for the production of bioplastics.


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