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    Discontinuous and continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 from renaturation batches.

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    Authors
    Gueorguieva, Ludmila
    Vallejo, Luis Felipe
    Rinas, Ursula
    Seidel-Morgenstern, Andreas
    Issue Date
    2006-12-01
    
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    Abstract
    Bone morphogenetic protein-2 (BMP-2) is one of the most interesting of the approximately 14 BMPs which belong to the transforming-growth-factor-beta (TGF-beta) superfamily. BMP-2 induces bone formation and thus plays an important role as a pharmaceutical protein. Recently, rhBMP-2 has been produced in form of inactive inclusion bodies in Escherichia coli. After solubilization and renaturation the biologically active dimeric form of rhBMP-2 can be generated. However, inactive monomers of BMP-2 are also formed during the renaturation process which must be separated from the active dimeric BMP-2. The purpose of this paper is to present: (a) results of an experimental study of a chromatographic separation of the monomeric and dimeric forms; and (b) a concept for a continuous counter-current simulated moving bed (SMB) process. The capacity of heparin as stationary phase was estimated for different salt concentrations in the mobile phase. A simulation study of a three-zone SMB process was performed applying a two step salt gradient. The results reveal the potential of the process for the purification of the dimeric BMP-2.
    Citation
    Discontinuous and continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 from renaturation batches. 2006, 1135 (2):142-50notJ Chromatogr A
    Affiliation
    Otto-von-Guericke-Universität Magdeburg, Institut für Verfahrenstechnik, PO Box 4120, D-39106 Magdeburg, Germany.
    Journal
    Journal of chromatography. A
    URI
    http://hdl.handle.net/10033/15836
    DOI
    10.1016/j.chroma.2006.08.061
    PubMed ID
    17064713
    Type
    Article
    Language
    en
    ISSN
    0021-9673
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.chroma.2006.08.061
    Scopus Count
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    Publications from Division of Molekulare Struktur Biologie (MOSB)

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